Validating TIM Cell: Bridge the gap between bioaccessibility & absorption

Conventional permeability assays use simplified buffer systems that fail to capture the complex intestinal environment. When drugs are administered to the TIM^® systems at clinically relevant doses, drugs are exposed to physiologically accurate intestinal fluids, containing bile salts, phospholipids, enzymes and digestion products, and both free and micelle-bound solubilized drug. Samples collected from the TIM^® lumen could be used for highly biorelevant permeability assays, particularly for drugs with poor solubility and permeability. However, key components of these biorelevant fluids, such as bile salts, are cytotoxic when applied to traditional Caco-2 monolayers, the gold-standard for permeability assays, limiting their use.

TIM Cell was developed to bridge the gap between bioaccessibility and absorption and bring permeability testing closer to physiological conditions